It’s a well accepted fact that weight training stimulates testosterone production, while certain types of cardiovascular training (especially HIIT) can do the same.
What is not often talked about, is the effect of training order and/or timing of the workouts, which is what this article should answer. In particular, we’re going to focus on two big questions:
- Does it matter for T production if you do cardio after or before weight training?
- Does it matter for T production if you train at AM or PM?
Here’s what research says:
Training Order and Timing for Optimal T Stimulation

…The “classic” cardio first, weights second order seems to be the best for hormone stimulation.
In fact, a study by Rosa et al. shows some very promising evidence for doing cardio first:
The researchers had 14 male subjects who were divided into 2 groups…
…The first group did 32 minutes of interval running on a treadmill before hitting the weights for a resistance training routine, consisting of various classic movements in manner of doing three sets of 10 reps at 70% of their 1-rep max.
The second group did the same stuff in reverse order, weight training first and then 32 minutes of interval running.
Immediately after the exercise regimen, the group that did cardio first had 7x higher serum testosterone levels (yes that’s not a typo, seven times…) The cardio first group had significantly higher post-exercise growth hormone levels too.
Why would the cardio first option be so much better? The researchers suggest that it’s all about stimulus interference that occurs if you do cardio after weights, and that sure does make sense.
NOTE: Post-exercise hormone fluctuations are not to be taken too seriously, since a) in many cases they quickly return back close to baseline and b) it takes constantly elevated hormone levels to actually cause significant differences in the rate of muscle growth. However, having 7x higher testosterone levels after your training sessions for multiple times a week, should over time – in my humble opinion – cause some major positive adaptations in the endocrine system.
Well what about the time of workouts then? For the sake of testosterone, is it better to train at AM or PM?
The answer is simple, it doesn’t matter. Common sense would suggest that morning training should be better since testosterone levels are highest in the morning. However, few studies have shown that over long-term period of time, resistance training increases testosterone levels, regardless of the time of the day (study, study).
Bottom line: Doing cardio before resistance training is significantly more “anabolic” than doing weights before cardio. These results however, are observed immediately post-exercise and likely fall back close to the baseline pretty quickly (hence, even though the increase in T is significant, it probably won’t do much for muscle growth). As for the time of your workouts, train whenever you feel like it, increases in testosterone occur similarly in the morning and evening.
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